Propagation of Ornamental Plants
24(1): 20-28, 2024
PHYSIOLOGICAL MECHANISMS OF 6-BENZYLAMINOPURINE IN THE REGULATION OF EMBRYOGENIC CALLUS PROLIFERATION IN AGAPANTHUS PRAECOX WILLD. SUBSP. ORIENTALIS (F. M. LEIGHT.) F. M. LEIGHT.
Changmei Du1, Haishuo Gao2, Yan Dong3, Hua Zhang1, Lei Liu1, Yun Teng1, and Jianhua Yue1*
1 School of Horticulture, Xinyang Agriculture and Forestry University, 1 Beihuan Road, Pingqiao District, 464100 Xinyang, China, *E-mail: jhyues@163.com 2 Xinyang Camellia oleifera Industry Development Center, 74 West Xinhua Road, Shihe District, 464000 Xinyang, China 3 School of Forestry, Xinyang Agriculture and Forestry University, 1 Beihuan Road, Pingqiao District, 464100 Xinyang, China
The proliferation of embryogenic callus (EC) is a crucial step in the rapid propagation of Agapanthus praecox Willd. subsp. orientalis (F. M. Leight.) F. M. Leight. by somatic embryogenesis (SE). The EC originating from the pedicle of A. praecox was employed as the test material. Then, the effects of 0.00 (control check, CK), 0.25, 0.50, and 1.00 mg l-1 6-benzylaminopurine (BAP) on EC proliferation were studied, and the physiological indices related to endogenous plant growth regulators (PGRs) metabolism, glycometabolism, and oxidative stress were researched. The addition of 0.25, 0.50, and 1.00 mg l-1 BAP promoted EC proliferation effectively, and the EC size significantly increased by 33.8%, 42.7%, and 44.1%, respectively, compared with that of the CK. The addition of 1.00 mg l-1 BAP to the medium significantly promoted the accumulation of endogenous indole-3-acetic acid (IAA), cytokinin (CTK), and gibberellins (GAs) and increased the activity of IAA oxidase. Moreover, the contents of total saccharide, sucrose, glucose, fructose, and hydrogen peroxide (H2O2) in EC decreased significantly, while the activity of catalase (CAT) increased significantly, indicating that BAP enhanced the degradation and utilization of saccharides and reduced the level of oxidative stress. The results indicate that BAP promoted EC proliferation by regulating endogenous PGRs metabolism, glycometabolism, and the intensity of oxidation, providing a reference for the optimization of the method for EC proliferation in A. praecox.
Key words: carbohydrate, cytokinin, endogenous PGRs, protective enzymes, reactive oxygen species, somatic embryogenesis
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