An efficient plant regeneration system involving callus induction, shoot regeneration and root formation was achieved in Dracaena sanderiana. Callus induction was best noted from nodal sections on Murashige and Skoog medium supplemented with 1.5 mg l^-1 2,4-dichlorophenoxyacetic acid (2,4-D). The nodal callus was later exposed to various levels of colchicine treatment for induction of mutants. The low levels of colchicine treatment (CT) improved callusing and callus biomass production in 0.10% (CT4; 3.3 g) and 0.05% (CT3; 3.1 g) after 7 weeks of culture compared to other treatments and the control (2.7 g). The calluses cultivated on medium supplemented with 1.75 mg l^-1 BAP showed high regeneration ability with 57.2 shoots at 0.03% (CT2), than treated with 0.01% colchicine (5.3 shoots). The number and length of regenerated shoots were relatively lower at higher levels of colchicine [CT4 (0.10%) - CT6 (0.20%)]. The analysis of protein
(19.9 mg g^-1 FW), amino acid (2.5 mg g^-1 FW), proline (39.0 mg g^-1 FW), and sugar (28.4 mg g^-1 FW) were high in callus of CT3 (0.05%) treatment, compared to values at the control regenerative callus. Next best condition was CT1 (0.01%), where the callus had enriched level of proteins, amino acids, proline and sugar. The rooting ability (96.2%) and the maximum number of roots were highest (25.16) on 1.5 mg l^-1 IBA added CT4 (0.10%) treatment. The regenerated plants were true-to-type and no apparent morphological variation was identified in colchicine-derived somaclones.