Aristolochia elegans is climbing shrub having beautiful duck shaped flowers and hence being used commonly in gardens. An in vitro clonal regeneration protocol was developed for this species through axillary bud proliferation. Aseptic nodal segments were cultured in Murashige and Skoog (MS) medium containing varying concentration of 6-benzylaminopurine (BAP) along with α-naphthaleneacetic acid (NAA) and kinetin (KIN). Maximum number of shoots (6.4) was obtained in MS medium supplemented with 4.0 mg l^-1 BAP + 0.5 mg l^-1 NAA with the longest shoot length (5.3 cm) also achieved in the same medium. Highest rate of multiplication (598%) was obtained in MS medium containing 1.0 mg l^-1 BAP and 0.5 mg l^-1 NAA. All the concentrations of Indole-3-butyric acid (IBA) (0.5 mg l^-1 and 0.75 mg l^-1 in combination with 50 mg l^-1 coumarin) resulted in 100% rooting. The rooted plantlets were successfully acclimatized with 75% survival in greenhouse for out-planting.