Dendrobium anosmum ‘Tim Hue’ is a local orchid from the Thua Thien Hue wild forest with typical color and fragrance, needing conservation measures. This study focuses on in vitro propagation of ‘Tim Hue’. Nodal segments used as initial explants were disinfected in 2% NaClO and 0.1% HgCl₂ for 10 min, resulting in 66.7% aseptic explants. Axillary shoot induction was best (88.9%) in Murashige and Skoog (MS) medium containing 1.5 mg l^-1 BAP and shoot multiplication was optimal in the same medium supplemented with 1.5 mg l^-1 BAP and 0.5 mg l^-1 IAA, with an estimated average of 6.0 shoots with 3.0 cm in length and 4.7 leaves after eight weeks of culture. Also, MS medium supplemented with 1 g l^-1 activated charcoal, and 0.7 mg l^-1 NAA or 0.5 mg l^-1 IAA was found the most efficient medium for the rooting of shoots. ‘Tim Hue’ plantlets were well acclimatized to the nursery conditions with a survival rate of 97.8% on coconut coir and rice husk coal (1 : 1, w : w) or 95.6% on fern board with sphagnum moss.