Pteris fauriei Hieron. was first reported as a new fern species for South Korea in 2014. Since then, propagation information for this species has not been clearly documented. Thus the aim of our study was to determine the in vitro spore germination conditions of P. fauriei and to suggest an efficient propagation method using mechanically fragmented gametophytes. Spore germination was tested under different light spectra (cool white fluorescent light (CWFL), red and blue light), temperature (15, 20, 25, and 30°C), and culture medium (Knop and one-eighth, quarter-, half-, full-, and double-strength Murashige and Skoog (MS)) conditions. Optimal conditions for the best germination (96.1%) were Knop medium, 25°C, and the CWFL. Blue light, low temperature, and double-strength MS medium conditions inhibited spore germination. Sporophyte production by applying the mechanical fragmentation method was done using gametophytes obtained from spore germination. This method produced a maximum of 114.5 sporophytes in full-strength MS medium in vitro. Therefore, we suggest mass production methods for P. fauriei seedlings, which can be easily implemented in the in vitro systems.