ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
19(3): 66-71, 2019

IN VITRO REGENERATION VIA CALLUS INDUCTION
IN DENDROCALAMUS ASPER (SCHULT.) BACKER

Qiaolu Zang, Qianqian Liu, Fei Zhuge, Xiaoqin Wang, and Xinchun Lin*

State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, 666 Wushu Road, Hangzhou, Zhejiang, 311300, China, *Fax: + 86 571 63743863,
*E-mail: linxcx@163.com



We developed an efficient plant regeneration system for culturing the plantlets of a valued ornamental bamboo species Dendrocalamus asper by using mature zygotic embryos as explants. The effects of different basal media and concentrations of 2,4-dichlorophenoxyacetic acid (2,4-D) on callus induction were analyzed. Murashige and Skoog (MS) basal medium supplemented with 3 mg l-1 or 0.5 mg l-1 2,4-D was suitable for callus formation and proliferation, respectively. In addition, MS medium supplemented with 2 mg l-1 N6-benzyladenine (BA), 1 mg l-1 kinetin, and 0.5 mg l-1 α-naphthaleneacetic acid was optimal for the callus differentiation and subsequent shoot growth, resulting in a higher differentiation up to 43.4%. The differentiation of callus subcultured twice was higher than that of callus subcultured more times. The root formation was 90% in the half-strength MS medium supplemented with 3 mg l-1 indole-3-butyric acid. Rooting plantlets showed a 95% survival when they were transferred and grew in the greenhouse.

Key words: bamboo, basal media, large-scale micropropagation, plant growth regulators, zygotic embryo



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