Propagation of Ornamental Plants
19(1): 24-29, 2019
SOMATIC EMBRYOGENESIS IN HOSTA MINOR (BAKER) NAKAI
Doo Hwan Kim and Iyyakkannu Sivanesan*
Department of Bioresources and Food Science, Institute of Natural Science and Agriculture, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 05029, Republic of Korea
*Fax: + 8224503310, *E-mail: email@example.com
An efficient in vitro propagation protocol via somatic embryogenesis of the most valuable crop Hosta minor has been developed. Anther filament and inflorescence segments were cultured on Murashige and Skoog (MS) medium augmented with 0, 2, 4, 6, 8 or 10 µM thidiazuron (TDZ) and 2, 4 or 6 µM α-naphthaleneacetic acid (NAA) to induce somatic embryogenesis. Among the two explants studied, anther filaments were found to be the most effective for somatic embryogenesis. The highest frequency of somatic embryo induction (95.6%) with a mean of 17.1 somatic embryos was obtained when anther filament explants cultured on MS medium with 8 µM TDZ and 2 µM NAA. The globular somatic embryos were transferred to the MS medium supplemented with 0, 1, 2, 4 or 8 µM gibberellic acid (GA3) for maturation and conversion. Somatic embryos converted best on MS medium with 2 µM GA3.
Key words: anther filaments, gibberellic acid, hosta, in vitro propagation, somatic embryo, thidiazuron