Two aspects of micropropagation of Narcissus tazetta, viz., contamination during initiation and multiplication were examined. A 30 min hot water treatment (HWT) of bulb segments at 40°C reduced contamination during initiation from 50% to less than 15%. After the HWT at 40°C, all explants survived and new shoot formation occurred in almost 100% of the explants, a noticeable increase relative to 70% in the nontreated control. The auxin transport inhibitor 2,3,5-triiodobezoic acid (TIBA) significantly (p < 0.01) increased shoot formation from twin scale explants. Fluridone, an inhibitor of strigolactone biosynthesis, increased shoot formation from scale explants a little. Newly formed shoots were rooted in vitro on medium with 0.5 µM indole-3-butyric acid (IBA). Plantlets with well-developed root and shoot systems were successfully acclimated (90%). They exhibited normal morphology and growth characteristics. Callus was initiated on different concentration of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP). In vitro tissues were screened for the production of alkaloids. In nondifferentiated callus, small amounts of galantamine occurred, which increased with tissue differentiation.