This study was focused on using nano-silver (nAg) as a biostimulator to improve the regenerative potential of three cultivars of lily: ‘Little John’, ‘Mona Lisa’, and ‘Osasco’. Bulb scales were soaked in aqueous solutions of nAg (0, 25, 50, 100, and 150 mg l^-1). nAg increased the number of bulblets at 50 mg l^-1 in the ‘Mona Lisa’ and ‘Osasco’, and enhanced the fresh weight of the bulblets and their roots, particularly at concentrations of 50 and 100 mg l^-1 in all researched cultivars.