ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
18(2): 39-48, 2018

REDUCTION OF HYPERHYDRICITY IN CARNATION BY SUPPLEMENTATION WITH RARE EARTH ELEMENTS DURING IN VITRO SHOOT MULTIPLICATION

Boling Liu1,3, Hao Wei2, Ji Eun Park2, and Byoung Ryong Jeong2*,3,4

1 College of Life Sciences, Qufu Normal University, 57 West Road Jingxuan, Qufu, 273165, Shandong, China
2 Division of Applied Life Science (BK21 Plus Program), Graduate School, Gyeongsang National University, 501 Jinju-daero str., 52828 Jinju, Republic of Korea,
*Fax: +82 55 757 7524, *E-mail: brjeong@gmail.com
3 Institute of Agriculture and Life Science, Gyeongsang National University, 501 Jinju-daero str., 52828 Jinju, Republic of Korea
4 Research Institute of Life Science, Gyeongsang National University, 501 Jinju-daero str., 52828 Jinju, Republic of Korea



Dianthus caryophyllus is an important ornamental plant globally. Tissue culture techniques have been used for the commercial production of carnation. However, micropropagation of carnation has been limited by the occurrence of hyperhydricity during shoot multiplication in vitro. The present study utilized rare earth elements (REEs) for reducing the incidence of hyperhydricity in micropropagated carnation during the multiplication stage. Nodal explants of D. caryophyllus ‘1130’ cultured in vitro for four weeks were cultured on the Murashige and Skoog medium containing 1.0 mg l-1 6-benzyladenine and 0.5 mg l-1 indole-3-acetic acid. The medium was supplemented with 0.05 or 0.1 mM of the REE in a form of lanthanum nitrate [La(NO3)3], cerium nitrate [Ce(NO3)3], or neodymium chloride (NdCl3). Hyperhydricity occurred in approximately 71.1% of the plantlets cultured using the medium devoid of REE. Among the REE treatments, the least incidence of hyperhydricity (48.3%) was observed in the medium supplemented with 0.05 mM Ce(NO3)3. Although no significant difference was found in accumulation of superoxide or hydrogen peroxide among treatments, reduction of hyperhydricity was correlated with lower activities of antioxidant enzymes, such as catalase, guaiacol peroxidase, and ascorbate peroxidase. Moreover, the total soluble protein content was increased in the 0.05 mM Ce(NO3)3 treatment. Therefore, less incidence of hyperhydricity by 0.05 mM Ce(NO3)3 seems to be caused by decreased oxidative stress during axillary shoot multiplication. These results suggest that 0.05 mM Ce(NO3)3 supplement could be an option for reducing incidence of hyperhydricity in micropropagated carnations in vitro.

Key words: antioxidant, micropropagation, oxidative stress, tissue culture



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