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Propagation of Ornamental Plants
16(3): 79-83, 2016
EFFECT OF META-TOPOLIN ON ADVENTITIOUS SHOOT REGENERATION IN
BEGONIA SEMIPARIETALIS Y. LIU, S. M. KU & C. I Peng
Tsai-Yu Chung1, Li-Peng Hsu1, and Wei-Hsin Hu2*
1 Department of Bioresources, Dayeh University, 168 University Rd. Dacun, 515 Changhua, Taiwan,
2 Department of Biology, National Museum of Natural Science, 1 Guancian Rd., 404 Taichung, Taiwan, *Fax: + 886-4-23285326, *E-mail: bohu@mail.nmns.edu.tw
Begonia semiparietalis Y. Liu, S. M. Ku & C.-I Peng, a recently discovered wild herb species with ornamental potential, was micropropagated. Laminae of in vitro plantlets with 5-7 mm2 in size were incubated on a half-strength Murashige and Skoog (MS) medium supplemented with 0.0-8.0 μM N6-benzylaminopurine, zeatin, or meta-topolin. After 8 weeks of culturing the highest frequency of adventitious shoot bud formation (36%), the maximum number of shoots (3.1), and shoot length of 0.4 cm was achieved with the supplement of 4.0 μM meta-topolin. The regenerated shoots were rooted (93.3%) on half-strength MS medium free of plant growth regulators.
Key words: conservation, cytokinin, micropropagation, ornamental plants
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