Propagation of Ornamental Plants
15(4): 142-146, 2015
MICROPROPAGATION OF SORBUS COMMIXTA HEDL.
Byoung Ryong Jeong1 and Iyyakkannu Sivanesan1,2*
1 Institute of Agriculture and Life Science, Department of Horticulture, Division of Applied Life Science (BK21 Plus), Gyeongsang National University, Jinju 660-701, Republic of Korea 2 Department of Molecular Biotechnology, Konkuk University, 1 Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea *Fax: + 8224503310 *E-mail: isivanesan@yahoo.com
Nodal explants obtained from greenhouse-grown plants of Sorbus commixta were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, 2.0 or 4.0 mg l-1 N6-benzyladenine (BA) or thidiazuron (TDZ), and 1.0 or 2.0 mg l-1 BA in combination with 0.5 and 1.0 mg l-1 indole-3-acetic acid (IAA) or α-naphthaleneacetic acid (NAA) for axillary shoot multiplication. The maximum frequency of shoot formation (78.1%) with a mean of 6.6 shoots was obtained on MS medium supplemented with 2.0 mg l-1 BA. Addition of IAA or NAA to BA-containing medium improved further the shoot induction response. The highest frequency of shoot induction (96%) with a mean of 16.2 shoots obtained when the nodal explants were cultured on MS medium supplemented with 2.0 mg l-1 BA and 0.5 mg l-1 NAA. The regenerated shoots were cultured on half-strength MS medium supplemented with 0, 0.5, 1.0, 2.0 or 4.0 mg l-1 IAA, indole-3-butyric acid (IBA) or NAA for root induction. Out of the three auxins studied, IBA was found to be the most effective in promoting root induction in S. commixta followed by NAA and IAA. The regenerated shoots were rooted best on half-strength MS medium supplemented with 2.0 mg l-1 IBA after 30 days of culture.
Key words: auxin, BA, nodal explants, Rosaceae, shoot multiplication, thidiazuron
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