Effect of plant growth regulators on axillary shoot multiplication from nodal explants of Ajuga multiflora was studied. Multiple shoots were induced on Murashige and Skoog (MS) medium containing N⁶-benzyladenine (BA) alone or in combination with indole-3-acetic acid (IAA) or in combination with α-naphthaleneacetic acid (NAA). The maximum shoot induction (79.9%), with a mean number of 9.3 shoots was obtained on MS medium supplemented with 2.0 mg l^-1 BA. A combination of BA and auxin was better than BA alone in promoting axillary shoot multiplication from the nodal explants of A. multiflora. The highest mean number of shoots (21.3) was obtained on MS medium supplemented with 2.0 mg l^-1 BA and 0.5 mg l^-1 NAA.