Propagation of Ornamental Plants
14(4): 184-190, 2014
MICROPROPAGATION OF AN INTERSPECIFIC HYBRID DOGWOOD (CORNUS ‘NCCH1’)
Jason Daniel Lattier, Darren Harvey Touchell*, and Thomas Green Ranney
Mountain Crop Improvement Lab, Department of Horticultural Science, Mountain Horticultural Crops Research and Extension Center, North Carolina State University, 455 Research Drive, Mills River, NC 28759-3423, USA *Fax: +1 828 684 8715, *E-mail: darren_touchell@ncsu.edu
A micropropagation protocol was developed for a novel hybrid dogwood, Cornus ‘NCCH1’ (an F2 hybrid of Cornus kousa Buerger ex Miq. ‘Miss Satomi’ × Cornus hongkongensis Hemsl. ‘Summer Passion’). Murashige and Skoog (MS) medium, woody plant medium (WPM), Driver and Kuniyuki Walnut (DKW) medium, Quoirin and Lepoivre (QL) medium, and Schenk and Hildebrandt (SH) medium, supplemented with 5 µM 6-benzylaminopurine (BAP), were evaluated as basal multiplication media. Benzylaminopurine, zeatin (Ztn), meta-Topolin (mT), or kinetin (Kin) at 0.625, 1.25, 2.5, 5, or 10 μM on WPM were investigated. Woody plant medium supplemented with 10 μM BAP provided the highest mean number of 4.27 axillary shoots (5-10 mm long) after 5 weeks of culture. The effect of supplementing multiplication media with indole-3-acetic acid (IAA) at 0, 0.1, 0.5, or 1.25 μM on shoot elongation was evaluated. The addition of IAA at 0.5 μM produced a 19% increase in mean shoot length per subsample over 5 weeks. The influence of media strength and sucrose concentration on rooting and ex vitro establishment was investigated. Quarter-strength WPM supplemented with 5 g l-1 sucrose produced the highest ex vitro establishment of 72.5%.
Key words: auxins, cytokinins, elongation, microshoots, multiplication rate, phenolics
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