The study focused on the effect of TDZ on axillary shoot multiplication from nodal explants of Sphagneticola trilobata. Nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.25, 0.5, 1.0 or 2.0 mg l^-1 TDZ, 3% (w/v) sucrose, and 0.8% (w/v) plant agar for shoot multiplication. The highest frequency of shoot induction (91.8%) and mean number of shoots (10.6) was obtained on MS medium supplemented with 0.5 mg l^-1 TDZ, while the greatest shoot length (4.6 cm) was recorded on MS medium containing 0.25 mg l^-1 TDZ. The number of subcultures had a significant effect on axillary shoot multiplication. The mean number of shoots and shoot length were significantly reduced in the third subculture. Furthermore, the regenerated shoots exhibited chlorosis on younger leaves in the third subculture. Regenerated shoots (> 3.0 cm long) were separated from the shoot clusters and cultured on half-strength MS medium supplemented with 0, 1.0, 2.0 or 4.0 mg l^-1 IBA for root induction. The highest frequency of root induction (100%) was obtained on half-strength MS medium supplemented with 2.0 or 4.0 mg l^-1 IBA. The greatest mean number of 8.6 roots with a mean root length of 6.3 cm was obtained on half-strength MS medium supplemented with 2.0 mg l^-1 IBA.