Propagation of Ornamental Plants
14(3): 125-132, 2014
EX VITRO ACCLIMATIZATION OF GLADIOLUS PLANTLETS
Enrique González-Pérez1*, Juana Juárez-Muñoz2, Oscar Javier Ayala-Garay3,
and María de Jesús Yáñez-Morales4*
1 Bajío Experimental Station, National Institute of Forestry, Agriculture, and Livestock, INIFAP, Km. 6.5 Highway, Celaya-San Miguel de Allende, P. O. Box 112, 38110 Celaya, Guanajuato State, México, *Fax: +52-461-611-5323, *E-mail: email@example.com
2 Hidalgo State University, 43660 Aquetzalpa, Tulancingo, Hidalgo State, México.
3 Genetics Resources Department, Postgraduate College-Campus Montecillo, 56230 Montecillo-Texcoco, México State, México.
4 Plant Pathology Department, Postgraduate College-Campus Montecillo, 56230
Montecillo-Texcoco, México State, México.
In Mexico the gladiolus (Gladiolus grandiflorus Andrews) asexual propagation is mainly carried out with corms and cormels produced by the farmers. This type of asexual reproduction is problematic when trying to obtain high quality corms because of the corm diseases spread more easily. Therefore, the aim of this study was to determine the most suitable environment for acclimatization of micropropagated Fusarium free plantlets. For in vitro propagation five different combinations of plant growth regulators were evaluated and for ex vitro acclimatization four environments were tested on a completely randomized experimental design. The highest rooted shoots percentage, number of roots, shoot length and 100% fungi-free plantlets were obtained when 0.2 mg l-1 NAA was used. The best acclimatization environment was under 75% shading mesh which promoted the highest survival (96.8%), plant length (24 cm), corm biomass (1.2 g), total biomass (2.5 g plant-1), and photosynthetic efficiency index (2.9). Additionally, the variable and maximum fluorescence ratio was nearly the optimal (0.83). This results could facilitate the large-scale production of Fusarium-free gladiolus plants without need be cultivated in a greenhouse conditions.
Key words: micropropagation, shading environment, Fusarium.