Propagation of Ornamental Plants
14(2): 68-75, 2014
DIRECT BULBLET REGENERATION FROM STERNBERGIA FISCHERIANA (HERB.) RUPR. BULB SCALE EXPLANTS
Suleyman Kizil1*, Khalid Mahmood Khawar2, Cigdem Altuntas1, and Sevil Saglam3
1 Dicle University, Faculty of Agriculture, Department of Field Crops, Ziraat Fakultesi str., 21280 Diyarbakir, Turkey, *Fax: + 90 41 22488153, *E-mail: suleymankizil@gmail.com 2 Ankara University, Faculty of Agriculture, Department of Field Crops, Kecioren Fatih str., 06110 Ankara, Turkey 3 Ahi Evran University, Faculty of Agriculture, Department of Agricultural Biotechnology, Asik Pasa Kampusu, Kayseri-Kirsehir road, 40000 Kirsehir, Turkey
Attractive golden yellow flowered Sternbergia fischeriana (Herb.) Rupr. multiplies very slow under natural conditions. The study reports multiplication of plants using 0.5, 1.0, and 1.5 cm long bulb scale explants with two, three, four, and five scales attached by a thin base plate segment. Any concentration of 2,4-D 1.0, 2.0, 3.0, 4.0, and 5.0 mg l-1 in MS medium was ineffective to induce bulblet regeneration on any explant at 15° ± 1°C. Discursive induction of one or two 0.1 cm diameter bulblets was noted at 24 ± 1°C on 0.5 cm long two-scale explants. Variable regeneration was observed on 0.5, 1.0, and 1.5 cm long two-scale explants on MS basal medium containing combination of BAP and 0.2 mg l-1 NAA. Maximum number of 5.0 ± 0.5 bulblets per 0.5 cm long two-scale bulb explant was obtained on MS medium containing 8.5 mg l-1 BAP plus 0.20 mg l-1 NAA. The rooting as affected by the size of bulblet was achieved on MS medium containing 0.75 mg l-1 NAA. The highest rooting was recorded on 0.47 cm diameter bulblets with 4.3 ± 0.9 roots per bulblet and 3.7 ± 0.4 cm long roots.
Key words: bulbous plant, in vitro micropropagation, rooting
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