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Propagation of Ornamental Plants
14(1): 26-31, 2014
GENETIC STABILITY DURING IN VITRO PROPAGATION OF DIANTHUS SPICULIFOLIUS SCHUR
Victoria Cristea1*, Cornelia CrДѓciunaЕџ2, Delia Marcu3, Magdalena Palada4, and Anca Butiuc-Keul2
1“BabeЕџ-Bolyai” University, “Alexandru Borza” Botanical Garden, 42 Republicii str., 400015 Cluj-Napoca, Romania, *Fax: + 40264-592152, *E-mail: victoria.cristea@ubbcluj.ro
2“BabeЕџ-Bolyai” University, Faculty of Biology and Geology, 5-7 Clinicilor str., 400006 Cluj-Napoca, Romania
3“BabeЕџ-Bolyai” University, Faculty of Environmental Science and Engineering, 33 Fântânele str., 400294 Cluj-Napoca, Romania
4Forest Research and Management Institute, Simeria Forest Research Station, 1 Biscaria str., 335900 Simeria, Romania
Dianthus spiculifolius Schur is an endemic and endangered plant species from Romania with high ornamental value. SSR markers were used to investigate the genetic structure of in situ populations of this species and also for study the genetic stability of in vitro plants. The molecular studies showed that the two populations are similar, and there are no specific markers of one population. The genetic variability of plants from natural habitats was conserved during in vitro culture. Genetic differences between somaclones derived from the same individual were low, so these plants can be used for ex situ collections and for ornamental purpose.
Key words: alleles frequency, gene diversity, heterozygosity, plant growth regulators, somaclonal variability, SSR markers
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