ISSN 1311-9109 Journal Content





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Propagation of Ornamental Plants
13(4): 189-195, 2013

CRYOPRESERVATION OF CALLERYA SPECIOSA (CHAMP.) SCHOT
THROUGH DROPLET-VITRIFICATION

Zhiying Li1, Tengmin Li2, Li Xu1*, and Bart Panis3

1 Institute of Tropical Crop Genetic Resources, Chinese Academy of Tropical Agricultural Sciences, Key Laboratory of South China Gene Resources and Germplasm Creating,
Ministry of Agriculture, Danzhou sity, Hainan province 571737, China,
*Fax: + 86 898 23300440, *E-mail: xllzy@263.net
2 Hainan University, Haikou, Hainan 571700, China
3 Bioversity International, Catholic University of Leuven, 3001 Leuven, Belgium



Cryopreservation is a practical method for the safe preservation of plant genetic resources. Impregnation with cryoprotective agents and the use of suitable explants are key factors for a successful procedure. It is, however, often difficult to work with “hairy” explants because of their high surface tension preventing an efficient treatment with cryoprotectant. Axillary shoot segments with one bud of Callerya speciosa were successfully cryopreserved by droplet-vitrification. The results showed that 1 mm shoot segments with one axillary bud excised from in vitro shoots could be used as explants for cryopreservation. The best pretreatment was performed with loading solution (LS), applied for 240 min at 25°C on a shaker at 150 rpm. This was followed by a plant vitrification solution (PVS2) treatment at 0°C for 60 min and rapid freezing in liquid nitrogen. Especially, rotation at 150 rpm proved to be essential to obtain high regrowth rates up to 60%.

Key words: axillary buds, hairy explant, shake



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