Propagation of Ornamental Plants
13(4): 181-188, 2013
IN VITRO SHOOT PROLIFERATION, ROOTING, AND ACCLIMATIZATION OF FOUR DIVERSE DIANTHUS PETRAEUS W. ET K. GENOTYPES USING TDZ, NAA, AND IBA
Georgios Tsoktouridis1*, Katerina Grigoriadou1, Evangelia Doua1, Anna Nikolaidou2, Georgios Menexes3, and Eleni Maloupa1
1 Laboratory for the Conservation and Evaluation of Native and Floricultural Species, Balkan Botanic Garden of Kroussia, Hellenic Agricultural Organization “Demeter”, P. O. Box 60125, 570 01 Thermi, Thessaloniki, Greece, *Fax: + 30 2310 478907,
2 Regional Laboratory for Agricultural Applications and Analysis of Fertilizers (P.E.G.E.A.L.) of Central Macedonia, Ministry of Rural Development and Food, Directorate of Research, Industrial Area, Sindos, 57400 Thessaloniki, Greece, 3Laboratory of Agronomy, School of Agriculture, Aristotle University, 541 24, Thessaloniki, Greece
In vitro mass propagation was achieved for four diverse genotypes of the wild carnation Dianthus petraeus, originating from four different geographical areas in Greece. Adventitious shoot proliferation was induced on Murashige and Skoog (MS) medium supplemented with 0.5, 1, and 2 mg l-1 of TDZ in combination with 0, 0.1 and 0.5 mg l-1 of NAA, using explant material from in vitro maintained stock microshoots. Hyperhydricity of the proliferated microshoots was observed and overcome by transferring the hyperhydrous microshoots to full-strength MS medium without growth regulators for four weeks prior to in vivo acclimatization. The effect of plant growth regulators was investigated and the mean number of adventitious shoots was recorded throughout all the developmental stages of micropropagation. The supplement of 0.1 mg l-1 NAA in the growth medium is recommended for all D. petraeus genotypes but treatments with different amounts of TDZ produced variable amounts of adventitious shoots (~17-24) per explant, depending on the genotype. A significant number of proliferated shoots was observed after the exclusion of TDZ and NAA during the hyperhydricity reduction treatment and a well developed root system formed using either NAA or IBA. The regenerated plantlets with well-developed root and shoot system were successfully acclimatized in greenhouse and exterior nursery conditions.
Key words: hyperhydricity, micropropagation, shoots proliferation, thidiazuron, wild carnation