ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
13(4): 168-173, 2013

IN VITRO PROPAGATION OF NANJING LINDEN (TILIA MIQUELIANA MAXIM.)

Naiwei Li1, Xiaochun Shu1, Shijie Tang1*, Gengguo Tang2, and Xiaoqing Lu1

1 Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, P. O. Box 1435,
1 Qianhu Houcun str., 210014 Nanjing, Jiangsu Province, China,
*Fax: + 86-25-84347006, *E-mail: tangshijie69@yahoo.com.cn
2 Nanjing Forest University, 159 Longpan Road, 210037 Nanjing, Jiangsu Province, China



Tilia miqueliana Maxim. is one of the most important tree species used in landscape architecture, especially in larger cities of China. Because of the high demand for this species, the development of an efficient micropropagation system can help to ensure an adequate supply of plants needed for city landscaping and reforestation. Seedlings were induced from seeds cultured on woody plant medium (WPM) without glycine, but supplemented with 8.66 µM Giberellic acid. Nodal segments with axillry buds were cultured on establishment WPM with 2.28 µM zeatin and 0.2 µM IBA. The resulting elongated axillary shoots were cut into nodal segments and transferred to shoot multiplication medium. The highest mean number of shoots (2.5) and nodes (4.5) was induced on WPM with 2.22 µM BA, 2.28 µM zeatin, and 0.1 µM IBA; while highest rooting (91.7%) was achieved on half-strength Murashige and Skoog medium with 14.76 µM IBA.

Key words: axillary bud, multiplication, nodal segment



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