Tilia miqueliana Maxim. is one of the most important tree species used in landscape architecture, especially in larger cities of China. Because of the high demand for this species, the development of an efficient micropropagation system can help to ensure an adequate supply of plants needed for city landscaping and reforestation. Seedlings were induced from seeds cultured on woody plant medium (WPM) without glycine, but supplemented with 8.66 µM Giberellic acid. Nodal segments with axillry buds were cultured on establishment WPM with 2.28 µM zeatin and 0.2 µM IBA. The resulting elongated axillary shoots were cut into nodal segments and transferred to shoot multiplication medium. The highest mean number of shoots (2.5) and nodes (4.5) was induced on WPM with 2.22 µM BA, 2.28 µM zeatin, and 0.1 µM IBA; while highest rooting (91.7%) was achieved on half-strength Murashige and Skoog medium with 14.76 µM IBA.