ISSN 1311-9109 Journal Content

International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy

Propagation of Ornamental Plants
13(4): 160-167, 2013


Abraham Arellano-Perusquía1, María Cristina Guadalupe López-Peralta2,
Francisco Chablé-Moreno3, and Andrés Adolfo Estrada-Luna1,4*

1 School of Agronomy, De La Salle Bajio University, 602 University Avenue, “Lomas del Campestre”, Leon, 37150 Guanajuato, Mexico, *Fax: + 052 (477) 7185511,
2 Laboratory of Biotechnology, Institute of Genetic Resources and Plant Productivity, Building of Genetics, Campus Montecillo, Graduate College, Montecillo,
56230 Estado de Mexico, Mexico
3 Tecnological Institute of Roque (CEPI-ITR), Kilometer 8, Highway Celaya-Juventino Rosas, P. O. Box 508, Celaya 38110 Guanajuato, Mexico 4Department of Genetic Engineering, Center for Research and Advanced Studies of the National Polytechnical Institute, Irapuato Unit, Kilometer 9.6 Northern Bypass, Highway Irapuato-Leon,
Irapuato, 36821 Guanajuato, Mexico

Ortegocactus macdougallii is highly appreciated ornamental and endemic Mexican cactus recently classified as a threatened plant species by the Government of Mexico. In order to rescue this plant from the risk of extinction and because conventional systems produce low propagation rates, we established a reliable and efficient method of micropropagation based in tubercle culture. During the establishment stage, varies concentracions of the combination of NAA (1.3, 4.4, and 13.3 µM) and BA (1.3, 4.4, and 13.3 µM) induced callus and shoot formation when explants (tubercles and axillary meristems or areoles dissected from adult plants) were incubated on Murashige and Skoog medium. In adition to this, the NAA free variants of the medium stimulated the rooting during the establishment stage, in particular the treatments including low and medium concentrations of BA (1.3 and 4.4 µM). During shoot proliferation, treatments including NAA (4.4 µM) and BA at 13.3, 19.9 or 26.6 µM produced between 5 and 6 shoots per explant; however, shoots obtained on medium with NAA 4.4 and BA 13.3 µM were statistically higher (5 mm). Rooting was produced on quarter- or half-strength MS medium but half-strength MS medium supplemented with IBA (1.6 or 4.9 µM) resulted in higher percent of rooted shoots (75%) and mean root number (8).

Key words: Cactaceae, cacti, in vitro propagation, micropropagation

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