ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
13(3): 138-145, 2013

IN VITRO GROWTH OF ALOE BARBADENSIS MILL.: THE EFFECT OF ACTIVATED CHARCOAL ON MEDIUM PH, NITROGEN UPTAKE AND ELEMENTS CONTENT OF SHOOTS

Daniela Borgognone1, Andrea Marcucci2, Giuseppe Colla1, Marco Renzaglia2,
Elvira Rea2, and Mariateresa Cardarelli2*

1 Department of Agriculture, Forestry, Nature and Energy, University of Tuscia, via San Camillo De Lellis, 01100 Viterbo, Italy
2 Agricultural Research Council - Research Centre for the Soil-Plant System, 00184 Roma, Italy, *Fax: + 39-(0) 6-7005711, *E-mail: mteresa.cardarelli@entecra.it



Activated charcoal was added to the elongation medium of Aloe barbadensis micropropagated in vitro. Its inclusion significantly increased shoot growth. In order to explain the improvement of the growth performance, the effect of activated charcoal on medium pH and elements uptake during the culture period was investigated. It was found that activated charcoal prevented the drop of the medium pH which occurred in its absence, probably because it decreased the ammonium:nitrate (NH4+ : NO3-) uptake ratio. In fact, a significant negative correlation between NH4+ : NO3- uptake ratio and medium pH occurred. Moreover, activated charcoal significantly affected the N, K, Ca, Na, Mn, Fe, B, and Zn concentration in the shoots, while days of culture influenced N, K, P, Na, Fe, Zn, and Cu presence in A. barbadensis shoots.

Key words: ammonium to nitrate ratio, micropropagation, nutrients uptake



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