ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
13(3): 130-137, 2013

DEVELOPMENT OF PLANT REGENERATION SYSTEM VIA SOMATIC EMBRYOGENESIS FROM ROOTS OF LILIUM HYBRID CULTIVARS

Yin Zhou1, Jianjun Zhang1, Minmin Chen1*, and Junjie Gu2

1 Forestry and Pomology Research Institute, Shanghai Academy of Agricultural Sciences, 1000 Jinqi Road, Fengxian District, 201403 Shanghai, China, *Fax: + 86 (0)21 62208157, *E-mail: cmm0707@163.com
2 Shanghai Flower Port Enterprise Development Co. Ltd., 2 Zhendong Road, Donghai Farm, Nanhui District, 201303 Shanghai, China



A simple and efficient protocol for in vitro plant regeneration through somatic embryogenesis was developed in four Lilium cultivars. Roots from in vitro seedlings of four lily cultivars were cultured on Murashige and Skoog (MS) medium (1962) supplemented with the auxin picloram (PIC) used alone (0.5, 1.0, and 2.0 mg l-1) or in combination with the cytokinins BAP or TDZ at 0.5 mg l-1. Picloram with the combination of cytokinins was found to be positive to improve the callus formation. Generally speaking MS medium supplemented with 1.0 mg l-1 PIC and 0.5 mg l-1 BAP gave the highest percentage of explants forming callus in three Lilium cultivars (‘Kankado’ = 95.7%; ‘Manissa’ = 98%; ‘Siberia’ = 80%); for ‘Sorbonne’ the best performance for callus formation was scored on MS medium + 1.0 mg l-1 PIC and 0.5 mg l-1 TDZ (88.7%). Embryoids regeneration occurred after a subsequent transfer onto fresh medium and a further one month of culture. The number of embryoids per 0.1 g of callus ranged from 11.7 (‘Siberia’ on MS medium + 1.0 mg l-1 PIC and 0.5 mg l-1 TDZ) to 70.3 (‘Manissa’ on MS medium + 1.0 mg l-1 PIC and 0.5 mg l-1 BAP). The callus induction efficiency, callus growth index, callus growth intensity, and numbers of embryoids induced from 0.1 g of callus of root were higher than that of bulb scales and leaves. Embryoids cultured on MS medium with 0.1 mg l-1 CPPU developed into complete plants.

Key words: cut flower, embryoids, embryogenic calluses, monocotyledonous, picloram, root system



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