Paulownia elongata is an ornamental, fast-growing species and resistant to urban conditions. It was successfully adapted to the growing conditions in Serbia. Twelve-year-old elite tree, originating from a plantation in Bela Crkva was used as stock plant for the experiments. In vitro culture was successfully established using disinfection of nodal segments of annual shoots with 0.1% HgCl₂ for 10 min. The most favorable shoot multiplication response (6.7 shoots per explant) was recorded on MS medium supplemented with 6 mg l^-1 BA and 0.5 mg l^-1 IBA, and rooting was successful (95%) on MS medium with 0.8 mg l^-1 of IBA and the same amount of NAA.