Amaranths have drawn a great interest in the last years as an agronomic crop, alternative energy source and ornamental plants around the world. Amaranthus cruentus L. ‘Ficha’ is the grain amaranth characterized with beautiful pink-yellow flowers shaped into big uniform inflorescence with the great potential for ornamental use. To develop in vitro propagation system – epicotyl, hypocotyls, root and leaf segments were used as initial explants from 10 day-old seedlings. The effect of BAP, zeatin, TDZ, and NAA were studied. The significantly highest adventitious shoot regeneration was observed on medium with 1 mg l^-1 TDZ without auxin using segments of epicotyl-derived shoots. The results indicate that the presence of TDZ in the culture medium could be essential for induction of morphogenic reaction in Amaranthus cruentus L.