In this study, effect of different inorganic micronutrients (CoCl₂, MnSO₄, ZnSO₄, CuSO₄ and H₃BO₃) on in vitro axillary shoots induction and proliferation in Dianthus caryophyllus L. ‘Fragrance Mix’ and D. chinensis L. ‘Double Flowered Mix’ was investigated. Shoots were induced directly from the cultured nodal segments without formation of intervening callus on MS medium supplemented with 4.40 µM BAP and 2.69 µM NAA and varying concentrations of micronutrients. Highest shoots induction was observed when the concentration of CuSO₄ in the medium was 1 µM (10 times higher than the MS level) and 0.5 µM (5 times higher than the MS level) for D. caryophyllus L. ‘Fragrance Mix’ and D. chinensis L. ‘Double Flowered Mix’, respectively. Shoots formed on induction medium were subcultured on medium with the same concentration of nutrients for proliferation. In proliferation medium highest number of axillary shoots 27 and 20 were formed at 1.0 µM CuSO₄ for D. caryophyllus ‘Fragrance Mix’ and D. chinensis ‘Double Flowered Mix’ respectively. Highest number of axillary shoots up to 17 was formed on medium with 0.55 µM CoCl₂ in D. caryophyllus ‘Fragrance Mix’ and 18 in D. chinensis ‘Double Flowered Mix’ on medium with 0.11 µM CoCl₂. A significant improvement in shoots induction and proliferation was observed, when the concentration of B, Zn, and Mn was higher in the medium. Random amplification of polymorphic DNA (RAPD) showed monomorphic bands in all the plants, thereby confirming clonal fidelity of the regenerants.