ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
13(1): 25-32, 2013

IN VITRO PROPAGATION OF AMSONIA ORIENTALIS DECNE.
FROM NODAL SEGMENTS OF ADULT PLANTS

Arda Acemi*, FazД±l Özen, and Ruhiye KД±ran

Department of Biology, Faculty of Sciences and Arts, Kocaeli University, 41380 Kocaeli, Turkey, *Fax: + 90 262 303 20 03, *E-mail: arda.acemi@kocaeli.edu.tr



Amsonia orientalis Decne. (syn. Rhazya orientalis (Decne.) A. DC.) is an ornamental and medicinal plant which has restricted distribution only in the northwest of Turkey and the northeast of Greece. Nodal segments excised from 7-year-old plants were cultured in MS medium supplemented with 0.5, 1.0, 2.0, 4.0 mg l-1 BAP for shoot multiplication. Medium containing 1.0 mg l-1 BAP suggested regeneration of the highest number of shoots (9.2 ± 0.2 mean number of shoots at 91.1 ± 2.3% frequency). Nodes obtained from multiplied shoots were excised and cultured in MS medium supplemented with combinations of BAP, kinetin, IAA, and 2,4-D. Maximum mean shoot length of 4.4 ± 0.7 cm was noted from the medium supplemented with 0.5 mg l-1 IAA + 1.5 mg l-1 BAP and 0.5 mg l-1 IAA + 0.5 mg l-1 kinetin individually. Rooting experiments were also conducted in half- and full-strength MS medium supplemented with IAA or IBA. All shoots could be induced to root on full-strength MS medium supplemented with 0.5 mg l-1 IAA within 4 weeks with a fairly good mean number and length of roots i.e. 7.9 ± 0.3 and 2.8 ± 0.2 cm. Plantlets went through a hardening phase in a controlled plant growth chamber prior to in vivo transfer. Eighty-four percent of the plantlets were succesfully acclimatized.

Key words: Apocynaceae, axillary shoot induction, blue star, in vitro rooting



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