In this communication, an efficient and reproducible protocol for in vitro flowering, fruiting and viable seed production of Ipomoea quamoclit L. is reported. The effect of cytokinins (BAP, KIN) on in vitro flower bud induction and the influences of carbon sources, viz., glucose, fructose, and sucrose, on in vitro fruiting and seed setting were compared at a wide range of concentrations (1.5-6%, w/v). Highest percentage of flowering (86.7 ± 3.3%) and maximum number flower buds per shoot (2.3 ± 0.14) were obtained on medium containing 0.3 mg l^-1 BAP. As a result of artificial in vitro pollination, highest (93.3 ± 4.4%) fruit production was obtained with medium containing 3.0% sucrose. Sucrose gave better fruit production (93.3 ± 4.4%) than glucose and fructose with 53.3 ± 3.3% and 36.7 ± 1.7%, respectively. Seeds produced on 4.5% sucrose containing medium showed highest 83.3 ± 3.3% germination. Within 89 days from the day of initial explant implantation in vitro flowering and fruiting were completed and the R₁ seeds were germinated to produce R₁ plants.