An efficient in vitro rooting system was developed for the endangered and difficult-to-root spring pasque flower (Pulsatilla vernalis (L.) Mill). Rooting of the shoots was observed on half-strength woody plant agar medium only with high concentration of IBA. Optimal rooting was observed for incised shoots grown on half-strength woody plant perlite medium with 20 mg l^-1 IBA for 3 weeks and then on an expressive medium. Under these conditions 84.7% of the shoots rooted. The incision of shoots (on the base of shoot) had significantly beneficial effects on root induction. Reduced induction phase running for 3 weeks significantly increased the rooting rate and a number of roots per explant. One-hour-dipping procedure into IBA solution and subsequent culture onto an expressive medium had little effect on rooting of shoots (16.7%). Application of auxin during in vitro rhizogenesis affected subsequent acclimatization of plantlets. The highest plantlet survival rate (90.5%) was observed in plants which were dipped in IBA and then exposured to the induction medium for 3 weeks during in vitro phase. The plants were replanted and transferred to a shady hotbed, and were ready for planting in the next year.