In this study, the morphogenesis of Cyclamen persicum Mill. using the thin cell layer technology (TCL) was successfully established. The peduncles were sliced into thin cell layers (1 mm thick), which were then cultured on MS medium supplemented with NAA, IBA, 2,4-D at various concentrations (0.1; 0.3; 0.5; 0.7 or 1.0 mg l^-1) alone or in combination with TDZ (0.2 mg l^-1). The results showed that the presence of auxin alone in variants of culture medium was not sufficient for callus induction from peduncle TCL explants. MS medium supplemented with 0.2 mg l^-1 TDZ and 1.0 mg l^-1 2,4-D was found to be the optimal medium for callus induction. Different sizes of thin cell layer explants (0.5, 1.0, 2.0 or 3.0 mm) from two positions of the peduncle (the upper or lower position of peduncle) were also studied. The 3 mm initiating explants at the upper position of the peduncle gave 100% callus induction rate and high callus fresh weight. After 8 weeks of culture, calluses which were transferred onto MS medium supplemented with 0.5 mg l^-1 BA and 0.7 mg l^-1 IBA induced the highest number of adventitious shoots (39.4 shoots/explant). Rooting reached 100% after transferring 2-3 cm long shoots to MS medium supplemented with 1.0 mg l^-1 IBA. The results indicated that different kinds of plant growth regulators, the explant size and position are important in determining the morphogenesis from peduncle TCL explants of Cyclamen persicum Mill.