Callus cultures of Bulbophyllum auricomum were successfully established from longitudinally bisected protocorm-like-bodies (PLB). Calluses were induced from PLB within one month of culture. Morphologically, two types of calluses (yellowish friable and yellowish green friable) were obtained from explants when cultured on basal medium supplemented with 2,4-D alone or combined with TDZ. The frequency of callus induction varied with concentrations and combinations of exogenously applied plant growth regulators; and hundred percent callus induction was achieved on MS basal medium supplemented with 1.0 mg l^-1 TDZ and 2.0 mg l^-1 2,4-D. The proliferated calluses maintained their totipotency for more than one year (through subcultures). Two types of morphologically distinct calluses (obtained via various callus induction treatments) were cultured on basal medium supplemented with 0.1 mg l^-1 TDZ for shoot regeneration. Hundred percent regeneration frequency and maximum shoot bud regeneration (46 shoot buds/100 mg callus) were observed in calluses induced on MS basal medium supplemented with 1.0 mg l^-1 TDZ and 0.5 mg l^-1 2,4-D (Callus line C10). PLB regeneration was observed in four callus lines producing shoot buds within one month, when transferred to fresh MS basal medium containing 0.1 mg l^-1 TDZ. The regenerated shoots from different calluses lines developed into complete plantlets when cultured on half-strength MS medium without any plant growth regulators.