For in vitro culture conservation via organ culture, mature embryos were excised from the seeds of Pinus armandii Franch. var. amamiana (Koidz.) Hatsushima. Shoots were cultured in different temperature conditions of 25, 20, 15, and 4°C to assess the effects on cool preservation of the culture. They could survive at least 5 months under each temperature. From the elongated shoots, root primordia and roots were induced on Root Induction Medium (RIM) medium containing 3 mg l^-1 IBA. Regenerated plantlets were grown in the field for three years. For long-term storage somatic embryogenic cells were cryopreserved in liquid nitrogen using the vitrification method. After thawing in 40°C water, the embryogenic cells were subcultured on Maruyama Embryo Maturation (EM) medium for recovery.