Ray florets of Chrysanthemum × grandiflorum (Ramat.) Kitam. were cultured in vitro on Murashige and Skoog (MS) medium containing various combinations and concentrations of BA and 2,4-D. The MS medium supplemented with 13.3 µM BA and 0.5 µM 2,4-D yielded the highest efficiency of callus formation after 1 month of culture. The calluses differentiated into multiple adventitious shoots. The highest number of regenerated shoots (45.0%) was obtained on MS medium supplemented with 9.3 µM kinetin and 4.9 µM IBA while the rooting of shoots reached 100% in all experiments. However, MS medium containing 12.3 µM IBA induced the highest number of roots per shoot (40.0). Histological observation showed that shoot formation originated from a single meristematic cell that had a dense cytoplasm and a well stained nucleus.