A protocol for in vitro plant multiplication of Cyrtopodium brandonianum from root-tip culture was developed. Root tips were dissected from 150 day-old plants, obtained in vitro and were cultured on half-strength MS medium, supplemented with different concentrations of BAP, kinetin, zeatin, tidiazuron, and 2iP for induction of adventitious shoots. After 60-70 days of incubation, shoot differentiation could be distinguished. The half-strength MS medium supplemented with 0.5 mg l^-1 TDZ showed the best response on shoot production, with, in average, 43% of the explants with shoots. Optimal rooting (20% of shoots with an average of 4.3 roots per explants), with no intervening callus was observed in medium containing half-strength MS medium, with 6% sucrose and 1 mg l^-1 NAA.