Propagation of Ornamental Plants
11(3): 156-158, 2011
INFLUENCE OF CYTOKININS ON ADVENTITIOUS SHOOT REGENERATION FROM LEAF AND PETIOLE EXPLANTS OF AJUGA MULTIFLORA BUNGE
Iyyakkannu Sivanesan1, Chung Ho Ko2, Jang Pyung Lee2, and Byoung Ryong Jeong1,2,3*
1 Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Korea 660-701 *Fax: + 82-55-757-7542, *E-mail: brjeong@gnu.ac.kr 2 Department of Horticulture, Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University, Jinju, Korea 660-701 3 Research Institute of Life Science, Gyeongsang National University, Jinju, Korea 660-701
Abstract
Direct adventitious shoots were initiated from leaf and petiole explants after 2 weeks of culture on Murashige and Skoog (MS) medium containing 0, 0.5, 1.0, 2.0, 4.0, 8.0, or 16.0 µM BA, 2iP or thidiazuron. Of the two explants used, petiole segments produced higher number of shoots per explant than leaf explant. Among the three cytokinins investigated, thidiazuron induced maximum number of shoots at 4.0 µM and the corresponding percentage of shoot induction was 100%. Lower concentrations of 2iP and BA did not initiate any shoots, while higher concentrations of TDZ induced callus and callus mediated regeneration. The regenerated shoots were transferred to MS medium and cultured for five weeks to induce the growth of shoots and roots. Shoot growth and root initiation were visible within 2 weeks following the transfer of shoots to MS medium and the frequency of root induction was 100% with a mean number of 8.2 roots per shoot. The in vitro-grown plantlets were successfully acclimatized in the greenhouse with 100% of survival.
Key words: Ajuga multiflora, cytokinins, direct regeneration, petiole explants, thidiazuron
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