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Propagation of Ornamental Plants
11(3): 144-148, 2011
STUDIES ON MULTIPLE SHOOT INDUCTION AND PROLIFERATION OF
PAEONIA LACTIFLORA PALL. ‘ZHONG SHENG FEN’
Xiao-Nan Yu1,2*, Hong Juan Wu1, Fang-Yun Cheng1,2, Jaime A. Teixeira da Silva3, and Miao-Miao Shen1
1 Beijing Forestry University, College of Landscape Architecture, 35 Qinghuadong Road, Beijing, 100083, China, *Fax: + 86 10 62 336063,
*E-mail: yuxiaonan626@126.com
2 National Engineering Research Center for Floriculture, 35 Qinghuadong Road,
Beijing, 100083, China
3 Faculty of Agriculture and Graduate School of Agriculture, Kagawa University,
Miki-cho, Ikenobe 2393, Kagawa-ken, 761-0795, Japan
Abstract
Underground buds of herbaceous peony (Paeonia lactiflora Pall.) ‘Zhong Sheng Fen’ were chosen as material for micropropagation in vitro. The basic process, including culture initiation, shoot induction and axillary shoot proliferation were established. The best initial medium for explant establishment was half-strength MS medium (CaCl2 in double strength) supplemented with 1 mg l-1 BAP plus 0.3 mg l-1 GA3. Resection flower buds and leaves could induce shoots. The best medium for shoot proliferation was half-strength MS medium (CaCl2 in double strength) supplemented with 0.2 mg l-1 BAP.
Key words: axillary shoots, Paeonia lactiflora, tissue culture, underground buds
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