The aim of this study was to assess inoculation and chemical disinfection of seeds and culture medium for in vitro propagation of Cattleya intermedia R. Graham, without the use of a laminar flow chamber. Two seed inoculation methods and six concentrations of sodium hypochlorite (NaClO) disinfectant (1.2, 12, 120, 1.200, 2.400, and 4.800 mg l^-1) were investigated comparatively against the conventional method, in which the seeds were surface-disinfected with NaClO (2.400 mg l^-1) and then rinsed in water in a laminar flow chamber. Two techniques for inoculating the culture medium with seeds were also investigated: a) using a syringe to inject seeds suspended in water into jars containing the culture medium, with a hole in the metal lids and a rubber seal, and b) using a beaker containing seeds suspended in water and then pouring the contents into jars with a metal lid and no hole or rubber seal. There was no contamination of the culture medium when NaClO was applied at concentrations ranging from 1.2 to 4.800 mg l^-1. Greater germination percentage (19% increase) was achieved by means of the syringe method when 1.2 mg l^-1 sodium hypochlorite (NaClO) solution was used. However, a lower number of plantlets (43% reduction) was achieved with beaker method. The fresh weight yield was higher in seedlings raised from the beaker method, especially at 1.2 (32%) and 120 mg l^-1 (335%) NaClO. The maximum fresh mass was achieved when seedlings were raised n the presence of 396 mg l^-1 NaClO, yielding average fresh mass of 5.0 g/jar, whereas control treatment produced 1.67 g/jar. It can be concluded that the disinfection using NaClO in solution is effective for in vitro germination and growth of the seedlings produced using the inoculation techniques, and that the syringe technique can be replaced by the beaker technique, which is more efficient and simpler to use.