|
Propagation of Ornamental Plants
10(3): 117-121, 2010
IN VITRO PROPAGATION OF CHAMAECYPARIS OBTUSA SIEB. ET ZUCC.
Ji Yun Min1, Dong Jin Park2, Mi Jin Jeong2, Hyun Jin Song2,
Yong Duck Kim3, Young Min Kang4, Chandrakant
Shivappa Karigar5, and Myung Suk Choi2*
1Sancheong Oriental Medicinal Herb Institute, Sancheong-gun, Gyeongnam,
666-831, South Korea
2Division of Environmental Forest Science, Gyeongsang National University, Jinju, 660-701, South Korea, *Fax: + 82-55-753-6015, *E-mail: mschoi@gnu.ac.kr
3Institute of Hadong Green Tea, Hadong-fun, Gyeongnam, 667-804, South Korea
4Department of Forestry, Mississippi State University, P. O. Box 9681, MS39762, USA
5Division of Biochemistry, Department of Chemistry, Central College Campus,
Bangalore University, Bangalore, 560-001, India
Abstract
Protocol for in vitro propagation of Chamaecyparis obtusa was established. Adventitious shoots were initiated from epicotyl explants after 4 weeks of culture on MS medium supplemented with 3% (w/v) sucrose and several concentrations of BAP. The effects of various nutritive media, carbon sources and several concentrations (4.44 to 22.20 μM) of BAP on in vitro shoot (upside of plantlet excepted roots) elongation and multiplication were determined. Optimal shoot growth was achieved on Woody Plant (WP) medium, they have over 25 shoots and 2.5 growth index (GI) after 8 weeks of culture. Among the carbon sources tested, 1% (w/v) fructose showed the highest length of the shoots (appr. 2.2 GI). The highest adventitious shoot induction was obtained on 4.44 μM of BAP, which gave 6.7 number of shoots after 8 weeks of culture and maximum shoot elongation was observed on WP basal medium without BAP which gave 7.8 cm long shoot 8 weeks after the transfer.
Key words: carbon source, Chamaecyparis obtusa, multiple shoot, propagation
|
