ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
10(2): 81-87, 2010

IN VITRO PROPAGATION OF LEONTOPODIUM ALPINUM CASS. FROM VARIOUS EXPLANTS OF SEEDLING

Alina Trejgell*, Dominika Szczepanek, Lucyna Domżalska,
and Andrzej Tretyn

Department of Biotechnology, Institute of General and Molecular Biology, Nicolaus Copernicus University, Gagarina 9, 87-100 Toruń, Poland, *Fax: + 48 56 611 4559,
*E-mail: trejgell@uni.torun.pl


Abstract
Shoot tips, fragments of cotyledons and hypocotyls were excised from 10-day-old sedlings and were used as initial explants. They were cultured on different variants of MS medium, containing BAP, kinetin, zeatin, and 2iP in combination with NAA for induction of shoots. The highest, frequency (100%) of axillary shoot proliferation was observed on shoot tips. BAP was the most effective cytokinin in axillary and adventitious shoot proliferation. An average of 15.1 shoots per explant were obtained on medium with 4.4 µM BAP in combination with 0.54 µM NAA, and the multiplication rate increased in four subsequent subcultures. The presence of BAP in the medium inhibited the elongation of shoots. The optimal effectiveness of rooting was observed when shoots were cultured on inductive MS medium containing half-strength nutrients and 5.4 μM NAA or 4.9 μM IBA for 2 weeks and then transferred to expressive medium (without auxin) for 4 weeks.

Key words: BAP, Leontopodium alpinum, proliferation, rooting



copyright (c) 2009, Sejani Publisher. All rights reserved.