ISSN 1311-9109 Journal Content





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CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
10(2): 59-66, 2010

IN VITRO REGENERATION OF LILIUM TSINGTAUENSE GILG. AND ANALYSIS OF GENETIC VARIABILITY IN MICROPROPAGATED PLANTS
USING RAPD AND ISSR TECHNIQUES

Wei-Ru Yang, Qi-Xiang Zhang*, Hui-Tang Pan, and Ming Sun

School of Landscape Architecture, P.O. Box 421, Beijing Forestry University, National Engineering Research Center for Floriculture, 35 Quinghua East Road, Haidian District Beijing, 100083 China, *Fax: + 86-10-62336126, *E-mail: zqx@bjfu.edu.cn


Abstract
Explants from bulb scales of Lilium tsingtauense Gilg. were cultured on MS medium with different exogenous plant growth regulators. M1 medium (MS + 0.1 mg l-1 NAA + 2.0 mg l-1 BA) produced 52 in vitro plantlets regenerated from adventitious shoots; M2 medium (MS + 0.1 mg l-1 NAA + 2.0 mg l-1 TDZ) had just 2 in vitro plantlets and M3 medium (MS + 2.0 mg l-1 BA+2.0 mg l-1 2,4-D) had none. These in vitro plantlets were examined for the presence of somaclonal variation by random amplified polymorphic DNA (RAPD) markers and inter simple sequence repeat (ISSR) markers. Twelve RAPD primers yielded 36 polymorphic bands and 10 ISSR primers produced 38 polymorphic bands, showing 36.73% and 43.18% polymorphism, respectively. Dendrograms based on polymorphism generated 70.8% (RAPD) and 73.7% (ISSR) similarity, indicating a low similarity index between each micropropagated plant and the donor plant. Both RAPD and ISSR markers were able to detect genetic variability during the regeneration process, but there was better reproducibility with ISSR markers.

Key words: ISSR, Lilium tsingtauense, micropropagation, RAPD, somaclonal variation



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