Plant regeneration was obtained from leaf explants of Lilium longiflorum by being cultured on MS medium supplemented with various concentrations of NAA (0.5, 1.0, 2.0 mg l^-1) and BAP (0.1, 0.5, 2.0 mg l^-1). The laminas showed higher bulblet regeneration ability than petioles, with the highest percentage of responsive explants on MS medium supplemented with 0.5 or 1.0 mg l^-1 NAA and 2.0 mg l^-1 BAP. Higher concentration of NAA resulted in excessive root formation. Direct bulblet regeneration was achieved on all variants of the medium studied. Bulblets and roots developed simultaneously on medium containing 0.5 or 2.0 mg l^-1 NAA and 0.1 mg l^-1 BAP without callus formation. Calluses appeared on BAP enriched medium, but soon developed into shoots or bulblets. The shoots or bulblets formed roots easily on MS medium containing 0.2 mg l^-1 NAA. Increasing the sucrose concentration from 3 g l^-1 to 12 g l^-1 resulted in higher efficiency of bulblet enlargement.