Appropriate micropropagation regimes were determined for the deciduous species Viburnum dentatum L. var. Lucidum Aiton. Shoot tip explants, collected from greenhouse grown plants and sterilized with 0.1% HgCl₂ and 1.2% NaOCl, were established on WPM with 10 μM BA. After two weeks, explants without any signs of contamination were subcultured on WPM, supplemented with BA, 2iP or kinetin at various concentrations (0, 5, 10, 20 μM), for multiple shoot formation. Explants produced more axillary shoots (average 2-4 shoots per explant) with BA than with 2iP or kinetin in a 4-week culture. After subculturing on fresh medium, elongated shoots were excised and induced to root on WPM supplemented with 0, 0.5, 2 or 8 μM IAA, IBA, or NAA. Lower concentrations (0.5 and 2 μM) of auxins were more effective than higher (8 μM) in stimulating rooting, at frequencies higher than 65% with 3-6 roots per shoot. Rooted shoots were transplanted to potting mixture and acclimatized for three weeks by transferring to environmentally controlled conditions. More than 90% of the potted microplants were successfully acclimatized and established in the greenhouse for further growth.