The major aim of this study was to establish the optimal parameters for rooting during micropropagation of Kazanlak oil-bearing rose (Rosa damascena Mill.) ‘Iskra’. Three series of experiment were conducted to study: I. The effect of the mineral composition of MS medium; II. The effect of auxins (IAA and NAA); III. The effect of the rooting conditions for cultivating the plantlets. A very high percentage of rooting (96.8%) with excellent viability of the microplants was achieved when they were grown on modified MS nutrient medium with macronutrients reduced to one fourth and 0.1 mg l^-1 NAA. The average number of roots formed and their average length were 5 roots and 13 mm per plant respectively. The fill up with liquid nutrient medium at the rooting stage promoted an increase of the number and the length of the induced rootlets as well as the growth of the stem part. The further grown plants of Kazanlak oil-bearing rose after autumn adaptation are an excellent planting material in the coming spring for establishing donor plantations or plantations for rose harvesting.