While Cyclamen persicum Miller has gained economic importance as an important ornamental plant worldwide, only a few of the other nineteen species of the same genus have commercial relevance although having a lot of interesting and valuable traits. To examine these traits under in vitro culture and greenhouse conditions an efficient in vitro propagation method of different Cyclamen species was established. Sterile seedlings of Cyclamen mirabile Hildebrand, Cyclamen coum Miller, Cyclamen graecum Link and Cyclamen hederifolium Aiton divided in cotyledon, tuber and roots were used as a starting material. Shoot induction was obtained on half-strength Murashige and Skoog based medium containing 0.5 mg l^-1 NAA and 1 mg l^-1 BAP and on medium with 0.5 mg l^-1 IAA, 1 mg l^-1 BAP, 1 mg l^-1 2iP and 1 mg l^-1 kinetin as growth regulators. The most suitable tissues for shoot induction were tubers and/or cotyledons depending on the species. Less or no shoots were obtained from roots when used as starting tissue, but root formation was found in high frequency. There were pronounced genotypic differences in the reaction to the different media within one species. The medium with lower contents of cytokinins showed high formation of roots, possibly due to high endogenous auxin content in the tissue.