Propagation of Ornamental Plants
8(3): 158-160, 2008
MICROPROPAGATION OF HIPPEASTRUM × CHMIELII CHM. FROM SCALE AND SCAPE EXPLANTS
Maria Witomska, Aleksandra Łukaszewska*, and Marcin Wojtowicz1*
Department of Ornamental Plants, Warsaw University of Life Sciences (WULS), Nowoursynowska 166, 02-787 Warszawa, Poland, *Tel./Fax: + 48 (22) 5932268, *E-mail: kro@sggw.pl
Abstract
An efficient propagation method is essential for rapid multiplication of a new hybrid. Here trials are described to micropropagate Hippeastrum × chmielii Chm. developed at WULS. Twin scales and sliced scapes were used as explants which were cultured on MS medium. The regeneration on scale explants occurred in absence of growth regulators while scape explants produced bulblets only when N6-benzyloadenine (BA) and α-naphtaleneacetic acid (NAA) were included in the medium. Only explants from 5-6 cm long scapes were able to differentiate bulblets; no bulblets were formed on explants from 10-12 cm long scapes. Absence of regeneration capability in longer scapes could be related to their higher contents of indole-3-acetic acid (IAA) and abscisic acid (ABA) as compared to shorter scapes.
Key words: ABA, Amaryllidaceae, Hippeastrum × chmielii, IAA, in vitro, scale and scape explants
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