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Propagation of Ornamental Plants
8(3): 154-157, 2008
ADVANCES IN THE MICROPROPAGATION OF SERVICE TREE (SORBUS DOMESTICA L.)
Paraskevas Nikolaou1, Dimitris Zagas1, Vassilis Scaltsoyiannes, Evangelos Balas3, Vassiliki Xilogianni3, Parthena Tsoulpha1, Maria Tsaktsira1, Eleni Voulgaridou1, Ivan Iliev2, Katerina Triantafyllou4, and Apostolos Scaltsoyiannes1*
1 Aristotle University of Thessaloniki, Department of Forestry and Natural Environment,
Laboratory of Forest Genetics and Plant Breeding, P.O. Box 238,
54124 Thessaloniki Greece, *Fax: + 302310992777,
*E-mail: skaltsoy@for.auth.gr
2 Department of Dendrology, University of Forestry, 10 Kliment Ohridski blvd.,
1756 Sofia, Bulgaria
3 Fytotechniki, Plant Tissue Culture Company, Filothei, 47042, Arta, Greece
4 Modagri Plants, Plants Cultivation & Selling Co, Modi 57014, Thessaloniki, Greece
Abstract
Nodal segments and shoot tips were used as explants, deriving from winter shoots of a mature Sorbus domestica tree (> 80 years old). Explants were cultured for micropropagation on MS medium supplemented with 4.44 μM BA and 0.05 μM IBA. Best proliferation and shoot elongation were achieved on MS media supplemented with 8.87 μM BA, 0.49 μM IBA, and 0.29 μM GA3. The use of red tinted filters further enhanced, both shoot proliferation and elongation. The best rooting results (33%) were obtained by microcutting quick-dip in 19.6 mM K-IBA and transfer for rooting on half strength MS medium. Our micropropagation protocol was applied by the private micropropagation company, "Fytotechniki", for large-scale prop agation of Sorbus domestica.
Key words: growth regulators, in vitro morphogenesis, large-scale propagation, micropropagation, Sorbus domestica
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