Differences in chlorogenic acid and rutin (quercetin-3-rutinoside) in different cutting parts (bases, stems and leaves) were observed immediately after severance in relation to the physiological stockplant status (mature, cutting, micropropagated) of ornamental cherry Prunus subhirtella 'Autumnalis'. The cuttings derived from the mature stockplants accumulated significantly lower concentrations of both substances during the first days after severance than the cuttings from the in vitro and cutting source. Moreover, the cuttings from the mature stockplants showed a rooting efficiency that was 32.3%, significantly worse than that for the cuttings of in vitro origin, with 76.7%. The cuttings harvested from cutting stockplants scored in the middle, with 56.7% rooting efficiency. The cutting stems contained the lowest concentrations of chlorogenic acid (up to 0.05 mg g^-1 FW) and rutin (up to 0.06 mg g^-1 FW), whereas the highest concentrations were measured in the cutting leaves, and, in the case of rutin, also in the cutting bases (up to 11 mg g^-1 FW chlorogenic acid and 5.5 mg g^-1 FW rutin).