The effect of Thidiazuron (TDZ) on multiple shoot formation in in vitro nodal explants of teak (Tectona grandis L.) was studied. Nodal explants were inoculated on MS medium supplemented with TDZ or BAP alone or in combination with IBA. TDZ at 8 μM concentration in the medium produced a 100% shoot induction response with 14.6 mean number of multiple shoots per culture vessel after 60 days. Similarly, multiple shoots were also obtained at comparatively lower concentration (1 μM) of TDZ but with smaller number of multiple shoots (8.3). TDZ’s influence on multiple shoot formation was better in all the tested combinations with BAP or IBA. However, once induced, shoot elongation was inhibited by all TDZ concentrations. Shoots were rooted in vitro within 2 week of culture on half-strength MS basal medium supplemented with 5, 10, 15, 20, or 25 μM each of IAA, IBA or NAA. Maximum rooting percentage (90%) with 2.8 mean number of roots and 2.70 cm mean root length was observed in medium with 25 μM IBA 35 days after the initial culture. After successful acclimatization, plants were transplanted to clay pots.