Propagation of Ornamental Plants
8(2): 111-113, 2008
IN VITRO MEDIUM-TERM CONSERVATION OF MYRTUS COMMUNIS L.
Maurizio Capuana* and Filomena Ponti
National Research Council, Plant Genetics Institute, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italy, *Fax: +39 (0) 55 5225729, *E-mail: maurizio.capuana@igv.cnr.it
Abstract
Clonal propagation of myrtle can be successfully achieved by micropropagation and, for many reasons related also to this, it would be of interest to make a method available for medium-term conservation of in vitro cultures. In our work we investigated the effect of low-temperature storage on the survival and proliferation capacity of microshoots. The influence of pre-treatments with abscisic acid and of conservation on mannitol-containing media were also investigated. It was found that myrtle can be easily stored for at least 6 months at 8ºC, recovering its proliferation capacity after this period.
Key words: abscisic acid, low temperature, mannitol, micropropagation, Myrtus communis, storage, tissue culture
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